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Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans

Rajabibazl, Masoumeh and Javad Rasaee, Mohammad and Nouri Fard, Mahtab and Farahnejad, Zohrea (2012) Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans. AJA.

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Abstract

Abstract Background: Candida albicans is one of the most common causes of hospital infections. The aim of this study was to develop a rapid, easy and sensitive way to detect candida albicans. Material and Methods: Polyclonal antibodies were prepared in rabbits. The antibodies were purified by salt concentration and Ion exchange chromatography. The purified antibody was coated onto the colloidal gold. Results: Color change (red to blue) was observed when the purified antigens (mannoprotein of Candida albicans cell wall) were added to Polyclonal antibodies. The method was sensitive and easy. Conclusion: This study indicated that using colloidal gold particle agglutination method can be used for accurate and rapid candida detection Method. Keywords: Candida Albicans, Mannoproteins, Antigen, Agglutination, Gold

Item Type: Article
Subjects: W Health professions
WX Hospital and other health Facilities
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: ePrint Admin Admin
Date Deposited: 14 Jul 2013 05:01
Last Modified: 14 Jul 2013 05:01
URI: http://eprints.ajaums.ac.ir/id/eprint/330

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