ClinicsOpen Access Volume 67, Issue 5, 2012, Pages 497-502

Acute and subchronic toxicological evaluation of Echinophora platyloba DC (Apiaceae) total extract in Wistar rats(Article)

  • aTehran University of Medical Sciences, Student Scientific Research Center (SSRC), Tehran, Iran
  • bAJA University of Medical Sciences, School of Medicine, Department of Physiology, Tehran, Iran
  • cKermanshah University of Medical Sciences, School of Pharmacy, Department of Pharmacology and Toxicology, Kermanshah, Iran
  • dKermanshah University of Medical Sciences, School of Pharmacy, Department of Pharmacognosy and Biotechnology, Kermanshah, Iran
  • eShaheed Beheshti University of Medical Sciences, School of Pharmacy, Department of Pharmacognosy, Tehran, Iran


Objective: Echinophora platyloba DC is a widely used herbal medicine and food seasoning in Iran. It is claimed to exert antimicrobial, antifungal, and antispasmodic effects. Despite the prevalent use of this plant as a food and medicine, there are no reports on its possible toxic effects. To evaluate the safety of E. platyloba, we tested its acute and sub-chronic toxicity in male and female Wistar rats. Methods: Rats were orally treated with four different single doses of E. platyloba total extract and screened for signs of toxicity two weeks after administration. In the sub-chronic toxicity study, E. platyloba was administered for 45 days. Mortality, clinical signs, body weight changes, hematological and biochemical parameters, gross findings, organ weights, and histological markers were monitored during the study. Results: We found no mortality and no abnormality in clinical signs, body weight, or necropsy findings in any of the animals in the acute study. The results of the subchronic study showed no significant difference in hematological parameters in either sex. There was a significant increase in lactate dehydrogenase in the female groups. A significant increase in the relative lung weight of female rats was noted at 500 mg/kg. Histopathological examinations revealed intra-alveolar hemorrhage in the male rats (500 mg/kg). In the females, congestion of the alveolar capillaries (at 500 mg/kg) and liver bridging necrosis (at 200 mg/kg) were significantly increased. Conclusion: The no observed adverse effect level of E. platyloba was determined to be 200 and 50 mg/kg for male and female rats, respectively. © 2012 CLINICS.

Author keywords

  • Acute toxicity
  • Echinophora platyloba
  • Rat
  • Subchronic toxicity

Indexed keywords

EMTREE drug terms:plant extract
EMTREE medical terms:animalApiaceaearticlebody weightcapillaryclassificationdose responsedrug effectdrug surveillance programfemaleliverlung alveolusmalemedicinal plantpathologyrattoxicity testingWistar rat
MeSH:AnimalsApiaceaeBody WeightCapillariesDose-Response Relationship, DrugFemaleLiverMaleNo-Observed-Adverse-Effect LevelPlant ExtractsPlants, MedicinalPulmonary AlveoliRatsRats, WistarToxicity Tests, AcuteToxicity Tests, Subchronic

Chemicals and CAS Registry Numbers:

Plant Extracts

  • ISSN: 18075932
  • Source Type: Journal
  • Original language: English
  • DOI: 10.6061/clinics/2012(05)15
  • PubMed ID: 22666795
  • Document Type: Article

  Hosseinzadeh, L.; Kermanshah University of Medical Sciences, School of Pharmacy, Department of Pharmacology and Toxicology, Iran; email:[email protected]
© Copyright 2013 Elsevier B.V., All rights reserved. © MEDLINE® is the source for the MeSH terms of this document.


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